Journal article

Microsatellite and minisatellite analysis of Leptosphaeria maculans in Australia reveals regional genetic differentiation

Helen L Hayden, Anton J Cozijnsen, Barbara J Howlett

PHYTOPATHOLOGY | AMER PHYTOPATHOLOGICAL SOC | Published : 2007

Abstract

ABSTRACT The population genetic structure of the fungal pathogen Leptosphaeria maculans was determined in Australia using six microsatellite and two minisatellite markers. Ascospores were sampled from Brassica napus stubble in disease nurseries and commercial fields in different sites over 2 years. The 13 subpopulations of L. maculans exhibited high gene (H = 0.393 to 0.563) and genotypic diversity, with 357 haplotypes identified among 513 isolates. Although the majority of genetic variation was distributed within subpopulations (85%), 10% occurred between the regions of eastern and Western Australia, and 5% within regions. F(ST) analysis of subpopulation pairs also showed the east-west gene..

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