Journal article

Phenotypic changes of morphologically identified guinea-pig myenteric neurons following intestinal inflammation

Kulmira Nurgali, Trung V Nguyen, Hayato Matsuyama, Michelle Thacker, Heather L Robbins, John B Furness

JOURNAL OF PHYSIOLOGY-LONDON | WILEY | Published : 2007

Abstract

We investigated the responses of morphologically identified myenteric neurons of the guinea-pig ileum to inflammation that was induced by the intraluminal injection of trinitrobenzene sulphonate, 6 or 7 days previously. Electrophysiological properties were examined with intracellular microelectrodes using in vitro preparations from the inflamed or control ileum. The neurons were injected with marker dyes during recording and later they were recovered for morphological examination. A proportion of neurons with Dogiel type I morphology, 45% (32/71), from the inflamed ileum had a changed phenotype. These neurons exhibited an action potential with a tetrodotoxin-resistant component, and a prolon..

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