Journal article
Analysis of the assembly profiles for mitochondrial- and nuclear-DNA-encoded subunits into complex I
Michael Lazarou, Matthew McKenzie, Akira Ohtake, David R Thorburn, Michael T Ryan
MOLECULAR AND CELLULAR BIOLOGY | AMER SOC MICROBIOLOGY | Published : 2007
DOI: 10.1128/MCB.00074-07
Abstract
Complex I of the respiratory chain is composed of at least 45 subunits that assemble together at the mitochondrial inner membrane. Defects in human complex I result in energy generation disorders and are also implicated in Parkinson's disease and altered apoptotic signaling. The assembly of this complex is poorly understood and is complicated by its large size and its regulation by two genomes, with seven subunits encoded by mitochondrial DNA (mtDNA) and the remainder encoded by nuclear genes. Here we analyzed the assembly of a number of mtDNA- and nuclear-gene-encoded subunits into complex I. We found that mtDNA-encoded subunits first assemble into intermediate complexes and require signifi..
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