Journal article

Expression of filtrin in human glomerular diseases

Pekka Ihalmo, Holger Schmid, Maria Pia Rastaldi, Deborah Mattinzoli, Robyn G Langham, Pauliina Luimula, Riika Kilpikari, Markus Lassila, Richard E Gilbert, Dontscho Kerjaschki, Matthias Kretzler, Harry Holthefer

NEPHROLOGY DIALYSIS TRANSPLANTATION | OXFORD UNIV PRESS | Published : 2007

Abstract

BACKGROUND: Filtrin (NEPH3/KIRREL2) is a recently characterized member of the nephrin-like proteins of the immunoglobulin superfamily, and it has been suggested to participate in the maintenance of the glomerular filtration barrier in the kidney. In this study, the gene and protein expression of filtrin were examined in patients with acquired proteinuric diseases. METHODS: Filtrin mRNA levels in renal biopsies were measured with quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) in two sets of patients with proteinuria. The mRNA levels were normalized to the housekeeping gene GAPDH and also related to the podocyte-specific genes nephrin and podocin. Immunofluorescence micr..

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