Journal article

Re-assessing the locations of components of the classical vesicle-mediated trafficking machinery in transfected Plasmodium falciparum

Akinola Adisa, Sarah Frankland, Melanie Rug, Katherine Jackson, Alexander G Maier, Peter Walsh, Trevor Lithgow, Nectarios Klonis, Paul R Gilson, Alan F Cowman, Leann Tilley

INTERNATIONAL JOURNAL FOR PARASITOLOGY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2007

Abstract

The malaria parasite, Plasmodium falciparum, exports proteins beyond the confines of its own plasma membrane, however there is debate regarding the machinery used for these trafficking events. We have generated transgenic parasites expressing chimeric proteins and used immunofluorescence studies to determine the locations of plasmodial homologues of the COPII component, Sar1p, and the Golgi-docking protein, Bet3p. The P. falciparum Sar1p (PfSar1p) chimeras bind to the endoplasmic reticulum surface and define a network of membranes wrapped around parasite nuclei. As the parasite matures, the endomembrane systems of individual merozoites remain interconnected until very late in schizogony. Ant..

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