Journal article

Transcriptional control of gluconeogenesis in Aspergillus nidulans

Michael J Hynes, Edyta Szewczyk, Sandra L Murray, Yumi Suzuki, Meryl A Davis, Heather M Sealy Lewis

GENETICS | GENETICS SOCIETY AMERICA | Published : 2007

Abstract

Aspergillus nidulans can utilize carbon sources that result in the production of TCA cycle intermediates, thereby requiring gluconeogenesis. We have cloned the acuG gene encoding fructose-1,6 bisphosphatase and found that expression of this gene is regulated by carbon catabolite repression as well as by induction by a TCA cycle intermediate similar to the induction of the previously studied acuF gene encoding phosphoenolpyruvate carboxykinase. The acuN356 mutation results in loss of growth on gluconeogenic carbon sources. Cloning of acuN has shown that it encodes enolase, an enzyme involved in both glycolysis and gluconeogenesis. The acuN356 mutation is a translocation with a breakpoint in t..

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