Journal article

Osteoclast inhibitory lectin (OCIL) inhibits osteoblast differentiation and function in vitro

Akira Nakamura, Chi Ly, Mirijana Cipetic, Natalie A Sims, Jessica Vieusseux, Vicky Kartsogiannis, Stellos Bouralexis, Hasnawati Saleh, Hong Zhou, John T Price, I John Martin, Ko Wah Ng ng, Matthew T Gillespie, Julian MW Quinn

BONE | ELSEVIER SCIENCE INC | Published : 2007

Abstract

Osteoclast inhibitory lectin (OCIL) is a type II C-type lectin and binds NK cell-associated receptor Nkrp1d and sulfated glycosaminoglycans. OCIL is expressed by several cell types found in bone and inhibits osteoclast differentiation. To determine whether OCIL may have wider effects on bone metabolism, we examined the effects of recombinant soluble OCIL on cultured osteoblasts and pre-osteoblastic KUSA O cells. Although OCIL did not affect osteoblast proliferation or apoptosis, or the formation of alkaline phosphatase positive colonies in cultured bone marrow, OCIL profoundly inhibited mineralization by primary osteoblasts and KUSA O cells in vitro. Analysis of ascorbate-treated KUSA O cell..

View full abstract