CCR5 Haplotypes and Mother-to-Child HIV Transmission in Malawi
Bonnie R Pedersen, Deborah Kamwendo, Melinda Blood, Victor Mwapasa, Malcolm Molyneux, Kari North, Stephen J Rogerson, Peter Zimmerman, Steven R Meshnick
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2007
BACKGROUND: CCR5 and CCR2 gene polymorphisms (SNPs) have been associated with protection against HIV transmission in adults and with delayed progression to AIDS. The CCR5 Delta32 deletion and SNP -2459G are associated with reduced expression of the CCR5 protein. METHODOLOGY/PRINCIPAL FINDINGS: We investigated the association between infant CCR2/CCR5 diplotype and HIV mother to child transmission (MTCT) in Malawi. Blood samples from infants (n = 552) of HIV positive women who received nevirapine were genotyped using a post-PCR multiplex ligase detection reaction and haplotypes were identified based on 8 CCR2/CCR5 SNPs and the open reading frame 32 base pair deletion. Following verification of..View full abstract
Awarded by NIH
Awarded by NIH-FIC
This work was supported by NIH grant # AI 49084 (SM), 5P01AI051649-05 (PZ), the NIH-FIC grant # 5 D43 TW00908 and the Center for AIDS Research at the University of North Carolina. SJR is a Wellcome Trust Senior Overseas Fellow in Biomedical Sciences. Pregnant women in this study were given nevirapine provided by Global Strategies for HIV Prevention. None of the sponsors had any input in the manuscript.