Journal article

High-level expression of a soluble and functional fibronectin type II domain from MMP-2 in the Escherichia coli cytoplasm for solution NMR studies

Alys A Peisley, Paul R Gooley

Protein Expression and Purification | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2007

DOI: 10.1016/j.pep.2006.12.005

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Keywords

Thioredoxins
Science & Technology
Escherichia Coli
Fibronectin Type Ii Domain
Biotechnology & Applied Microbiology
Iv Collagenase
Disulfide Bonds
Biochemistry & Molecular Biology
Mmp-2
Growth-Factor
Genetic Vectors
Matrix Metalloproteinase 2
Nuclear Magnetic Resonance, Biomolecular
Protein Structure, Tertiary
Cytoplasm
Matrix Metalloprotease
Module Pair
Fibronectins
Life Sciences & Biomedicine
(6)f1(1)f2(2)f2 Fragment
Thioredoxin
Chromatography, Affinity
Solubility
Protein Folding
Isotope Labeling
Human Matrix Metalloproteinase-2
Recombinant Fusion Proteins
Gelatin-Binding
Disulfides
Gene Expression
Biochemical Research Methods
Sequence
Molecular-Cloning
Histidine