Journal article

Genetic model for the chronic activation of skeletal muscle AMP-activated protein kinase leads to glycogen accumulation

Laura Barre, Christine Richardson, Michael F Hirshman, Joseph Brozinick, Steven Fiering, Bruce E Kemp, Laurie J Goodyear, Lee A Witters

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | AMER PHYSIOLOGICAL SOC | Published : 2007

Abstract

The AMP-activated protein kinase (AMPK) is an important metabolic sensor/effector that coordinates many of the changes in mammalian tissues during variations in energy availability. We have sought to create an in vivo genetic model of chronic AMPK activation, selecting murine skeletal muscle as a representative tissue where AMPK plays important roles. Muscle-selective expression of a mutant noncatalytic gamma1 subunit (R70Qgamma) of AMPK activates AMPK and increases muscle glycogen content. The increase in glycogen content requires the presence of the endogenous AMPK catalytic alpha-subunit, since the offspring of cross-breeding of these mice with mice expressing a dominant negative AMPKalph..

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University of Melbourne Researchers