Journal article

Isolated Anxa5( )/Sca-1( ) perivascular cells from mouse meningeal vasculature retain their perivascular phenotype in vitro and in vivo

Bent Brachvogel, Friederike Pausch, Peter Farlie, Udo Gaipl, Julia Etich, Zhigang Zhou, Trevor Camerone, Klaus von der Mark, John F Bateman, Ernst Poeschl

EXPERIMENTAL CELL RESEARCH | ELSEVIER INC | Published : 2007

Abstract

Pericytes are closely associated with endothelial cells, contribute to vascular stability and represent a potential source of mesenchymal progenitor cells. Using the specifically expressed annexin A5-LacZ fusion gene (Anxa5-LacZ), it became possible to isolate perivascular cells (PVC) from mouse tissues. These cells proliferate and can be cultured without undergoing senescence for multiple passages. PVC display phenotypic characteristics of pericytes, as they express pericyte-specific markers (NG2-proteoglycan, desmin, alphaSMA, PDGFR-beta). They also express stem cell marker Sca-1, whereas endothelial (PECAM), hematopoietic (CD45) or myeloid (F4/80, CD11b) lineage markers are not detectable..

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