Journal article

The Golgin GCC88 is required for efficient retrograde transport of cargo from the early endosomes to the trans-Golgi network

Zi Zhao Lieu, Merran C Derby, Rohan D Teasdale, Charles Hart, Priscilla Gunn, Paul A Gleeson

MOLECULAR BIOLOGY OF THE CELL | AMER SOC CELL BIOLOGY | Published : 2007

Abstract

Retrograde transport pathways from early/recycling endosomes to the trans-Golgi network (TGN) are poorly defined. We have investigated the role of TGN golgins in retrograde trafficking. Of the four TGN golgins, p230/golgin-245, golgin-97, GCC185, and GCC88, we show that GCC88 defines a retrograde transport pathway from early endosomes to the TGN. Depletion of GCC88 in HeLa cells by interference RNA resulted in a block in plasma membrane-TGN recycling of two cargo proteins, TGN38 and a CD8 mannose-6-phosphate receptor cytoplasmic tail fusion protein. In GCC88-depleted cells, cargo recycling was blocked in the early endosome. Depletion of GCC88 dramatically altered the TGN localization of the ..

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