Journal article

Sedimentation velocity analysis of amyloid oligomers and fibrils

Yee-Foong Mok, Geoffrey J Howlett, I Kheterpal (ed.), R Wetzel (ed.)

AMYLOID, PRIONS, AND OTHER PROTEIN AGGREGATES, PT C | ELSEVIER ACADEMIC PRESS INC | Published : 2006

Abstract

The different aggregation states of amyloid oligomers and fibrils have been associated with distinct biological properties and disease pathologies. These various amyloid species are distinguished by their different molecular weights and sedimentation coefficients and can be consistently resolved, separated, and analyzed using sedimentation velocity techniques. We first describe the theoretical background and use of the preparative ultracentrifuge to separate amyloid fibrils and their oligomeric intermediates from monomeric subunits as well as the factors and limits involved in such methods. The approach can be used to monitor the kinetics of fibril formation as well as providing purified fra..

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