Journal article

Hypertrophy and physiological death of equine chondrocytes in vitro

YA Ahmed, L Tatarczuch, CN Pagel, HM Davies, M Mirams, EJ Mackie

EQUINE VETERINARY JOURNAL | WILEY-BLACKWELL | Published : 2007

Abstract

REASON FOR PERFORMING STUDY: Equine osteochondrosis results from a failure of endochondral ossification during skeletal growth. Endochondral ossification involves chondrocyte proliferation, hypertrophy and death. Until recently no culture system was available to study these processes in equine chondrocytes. OBJECTIVE: To optimise an in vitro model in which equine chondrocytes can be induced to undergo hypertrophy and physiological death as seen in vivo. METHODS: Chondrocytes isolated from fetal or older (neonatal, growing and mature) horses were cultured as pellets in 10% fetal calf serum (FCS) or 10% horse serum (HS). The pellets were examined by light and electron microscopy. Total RNA was..

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