Journal article

Differential protein binding of indinavir and saquinavir in matched maternal and umbilical cord plasma

Sreeja Sudhakaran, Craig R Rayner, Jian Li, David CM Kong, Neil M Gude, Roger L Nation

BRITISH JOURNAL OF CLINICAL PHARMACOLOGY | BLACKWELL PUBLISHING | Published : 2007

Abstract

AIMS: To determine whether lower umbilical cord than maternal binding of indinavir and saquinavir contributed to the low cord : maternal (C : M) total concentration ratios reported previously. METHODS: Indinavir and saquinavir unbound fraction (fu) was determined using equilibrium dialysis. Buffer solutions of human serum albumin (HSA) (20.0, 30.0, 40.0 g l(-1)) and alpha(1)-acid glycoprotein (AAG) (0.20, 0.60, 2.00 g l(-1)) were spiked with indinavir (1.00 and 8.00 mg l(-1)) or saquinavir (0.15 and 1.50 mg l(-1)). Matched maternal and umbilical cord plasma was spiked with 1.00 mg l(-1) indinavir (n = 12) or 0.15 mg l(-1) saquinavir (n = 20). Spiked protein/plasma solutions were dialyzed aga..

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