Journal article

beta 2-microglobulin as a biomarker in peripheral arterial disease - Proteomic profiling and clinical studies

Andrew M Wilson, Eiichiro Kimura, Randall K Harada, Nandini Nair, Balasubramanian Narasimhan, Xiao-Ying Meng, Fujun Zhang, Kendall R Beck, Jeffrey W Olin, Eric T Fung, John P Cooke



BACKGROUND: Peripheral arterial disease (PAD) is common but commonly unrecognized. Improved recognition of PAD is needed. We used high-throughput proteomic profiling to find PAD-associated biomarkers. METHODS AND RESULTS: Plasma was collected from PAD patients (ankle brachial index of <0.90; n=45) and subjects with risk factors but without PAD (n=43). Plasma was analyzed with surface-enhanced laser desorption/ionization time-of-flight mass spectrometry to quantify 1619 protein peaks. The peak intensity of a 12-kDa protein was higher in PAD patients. Western blot analyses and immunoaffinity studies confirmed that this protein was beta2-microglobulin (B2M). In a validation study, B2M was measu..

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