Journal article

Mucosal HIV-1 pox virus prime-boost immunization induces high-avidity CD8( ) T cells with regime-dependent cytokine/granzyme B profiles

Charani Ranasinghe, Stephen J Turner, Craig McArthur, Duncan B Sutherland, Jee-Hye Kim, Peter C Doherty, Ian A Ramshaw

JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 2007

Abstract

The quality of virus-specific CD8(+) CTL immune responses generated by mucosal and systemic poxvirus prime-boost vaccines were evaluated in terms of T cell avidity and single-cell analysis of effector gene expression. Intranasal (I.N.) immunization regimes generated higher avidity CTL responses specific for HIV K(d)Gag(197-205) (amino acid sequence AMQMLKETI; H-2K(d) binding) compared with i.m. immunization regime. Single-cell RT-PCR of K(d)Gag(197-205)-specific mucosal and systemic CTL revealed that the cytokine and granzyme B expression profiles were dependent on both the route and time after immunization. The I.N./i.m.-immunized group elicited elevated number of CTL-expressing granzyme B ..

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