Journal article

Probing the flexibility of the DsbA oxidoreductase from Vibrio cholerae - a N-15-H-1 heteronuclear NMR relaxation analysis of oxidized and reduced forms of DsbA

James Horne, Edward J d'Auvergne, Murray Coles, Tony Velkov, Yanni Chin, William N Charman, Richard Prankerd, Paul R Gooley, Martin J Scanlon

JOURNAL OF MOLECULAR BIOLOGY | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2007

Abstract

We have determined the structure of the reduced form of the DsbA oxidoreductase from Vibrio cholerae. The reduced structure shows a high level of similarity to the crystal structure of the oxidized form and is typical of this class of enzyme containing a thioredoxin domain with an inserted alpha-helical domain. Proteolytic and thermal stability measurements show that the reduced form of DsbA is considerably more stable than the oxidized form. NMR relaxation data have been collected and analyzed using a model-free approach to probe the dynamics of the reduced and oxidized states of DsbA. Akaike's information criteria have been applied both in the selection of the model-free models and the dif..

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