Journal article

Structure of the Janus protein human CLIC2

Brett A Cromer, Michael A Gorman, Guido Hansen, Julian J Adams, Marjorie Coggan, Dene R Littler, Louise J Brown, Michele Mazzanti, Samuel N Breit, Paul MG Curmi, Angela F Dulhunty, Philip G Board, Michael W Parker

JOURNAL OF MOLECULAR BIOLOGY | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2007

Abstract

Chloride intracellular channel (CLIC) proteins possess the remarkable property of being able to convert from a water-soluble state to a membrane channel state. We determined the three-dimensional structure of human CLIC2 in its water-soluble form by X-ray crystallography at 1.8-A resolution from two crystal forms. In contrast to the previously characterized CLIC1 protein, which forms a possibly functionally important disulfide-induced dimer under oxidizing conditions, we show that CLIC2 possesses an intramolecular disulfide and that the protein remains monomeric irrespective of redox conditions. Site-directed mutagenesis studies show that removal of the intramolecular disulfide or introducti..

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