Journal article

Substrate utilization in porcine embryos cultured in NCSU23 and G1.2/G2.2 sequential culture media

AP Gandhi, M Lane, DK Gardner, RL Krisher

MOLECULAR REPRODUCTION AND DEVELOPMENT | WILEY | Published : 2001

Abstract

Embryo metabolism is an indicator of viability and, therefore, efficiency of the culture medium. Currently, little is known regarding porcine embryo metabolism. The objective of our study was to evaluate glucose and pyruvate uptake and lactate production in porcine embryos cultured in two different media systems. Oocytes were matured and fertilized according to standard protocols. Embryos were allocated randomly into two culture treatments, NCSU23 medium or G1.2/G2.2 sequential culture media 6-8 h post-insemination (hpi). Embryo substrate utilization was measured at the two-cell (24-30 hpi), 8-cell (80 hpi), morula (120 hpi), and blastocyst (144 hpi) stages using ultramicrofluorimetry. Gluco..

View full abstract

University of Melbourne Researchers