Granulocyte colony-stimulating factor and an RARα specific agonist, VTP195183, synergize to enhance the mobilization of hematopoietic progenitor cells

Abstract

BACKGROUND. Failure to mobilize adequate numbers of hematopoietic stem and progenitor cells (HSPC) is an important clinical problem. Since bone marrow (BM) neutrophils play a central role in HSPC mobilization, we hypothesized that granulocyte colony-stimulating factor (G-CSF)-mediated mobilization would be enhanced by further expanding the size of the BM granulocyte pool. METHODS. We tested the potential of the retinoic acid receptor alpha (RARα) specific agonist VTP195183, and the pan-RAR agonist all-trans retinoic acid (ATRA), to enhance G-CSF-mediated mobilization of HSPC, in two mouse strains. RESULTS. Pretreatment of mice with VTP195183 significantly increased the number of leukocytes, ..