Journal article

Hemopoietic stem cells with higher hemopoietic potential reside at the bone marrow endosteum

David N Haylock, Brenda Williams, Hayley M Johnston, Mira CP Liu, Kate E Rutherford, Genevieve A Whitty, Paul J Simmons, Ivan Bertoncello, Susan K Nilsson

STEM CELLS | WILEY | Published : 2007

Abstract

It is now evident that hemopoietic stem cells (HSC) are located in close proximity to bone lining cells within the endosteum. Accordingly, it is unlikely that the traditional method for harvesting bone marrow (BM) from mice by simply flushing long bones would result in optimal recovery of HSC. With this in mind, we have developed improved methodologies based on sequential grinding and enzymatic digestion of murine bone tissue to harvest higher numbers of BM cells and HSC from the endosteal and central marrow regions. This methodology resulted in up to a sixfold greater recovery of primitive hemopoietic cells (lineage(-)Sca(+)Kit(+) [LSK] cells) and HSC as shown by transplant studies. HSC fro..

View full abstract