The impact of constitutive heterologous expression of a moss Na transporter on the metabolomes of rice and barley

Abstract

The metabolic profiles of rice and barley plants constitutively expressing a sodium-pumping ATPase (PpENA1) isolated from the bryophyte Physcomitrella patens were examined using GC-MS. Quantitative real-time PCR (qRT-PCR) was used to determine the mRNA levels of PpENA1 in root and leaf tissues of the transgenic rice and barley lines. PpENA1 mRNA levels were significantly higher in rice lines than in barley lines with the same dual CaMV35S promoter controlling PpENA1 transcription in both species. In rice, PpENA1 mRNA levels were greatest in the shoot whilst levels were greatest in the roots of barley. Metabolite profiles were determined in the flag leaf of both rice and barley plants grown u..