Journal article
Recombinant human albumin supports hamster in-vitro fertilization
BD Bavister, DL Kinsey, M Lane, DK Gardner
HUMAN REPRODUCTION | OXFORD UNIV PRESS | Published : 2003
Abstract
BACKGROUND: Serum albumin is normally required to support sperm capacitation and IVF, but its mechanism of action is not well understood. Commercial serum albumin preparations are contaminated with a variety of other proteins and compounds, and their biological activity is variable. Recombinant human albumin (rHA) might replace serum albumin for IVF. METHODS: rHA was examined for its ability to capacitate hamster spermatozoa and to support fertilization in vitro. A standardized hamster IVF system was used to compare the capacitation-supporting activities of rHA and two commercial preparations of bovine serum albumin (BSA) in a chemically defined culture medium. Epididymal spermatozoa were in..
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