Oestradiol, cyclodextrin-encapsulated 17β-oestradiol and the oestradiol solubilizer 2-hydroxypropyl-β-cyclodextrin all impair preimplantation mouse embryo development

Abstract

The aim of this study was to examine the effects of 2-hydroxypropyl-β-cyclodextrin (HβC) used as a solubilizer for oestradiol, 17β-oestradiol (ethanol soluble) and HβC-encapsulated-17β-oestradiol on mouse embryo development in vitro. HβC had no effect on day 3 development. In contrast, blastocyst development and blastocyst cell number were significantly reduced in the presence of 10-4 mol/l solubilizer equivalent, but not at lower concentrations. The proportion of compacted embryos was significantly reduced with 10-4 mol/l 17β-oestradiol. No blastocysts were formed at 10-4 mol/l concentration of 17β-oestradiol, although the rate of blastocyst formation did not differ at lower concentrations...