Patient and sample-related factors that effect the success of in vitro isolation of Orientia tsutsugamushi.
Rungnapa Luksameetanasan, Stuart D Blacksell, Thareerat Kalambaheti, Vanaporn Wuthiekanun, Wirongrong Chierakul, Sunee Chueasuwanchai, Apichat Apiwattanaporn, John Stenos, Stephen Graves, Sharon J Peacock, Nicholas PJ Day
Southeast Asian J Trop Med Public Health | Published : 2007
Orientia tsutsugamushi is the causative agent of scrub typhus infection, a major cause of human disease in rural areas of Southeast Asia. Twenty-six blood samples collected from patients with serologically proven scrub typhus during a six month period were sent to Bangkok (535 km from the clinical site) by road at ambient temperature (average daily temperature range: 27.1-29.1 degrees C) for attempted in vitro isolation in Vero cells. O. tsutsugamushi was isolated from 12 samples (sensitivity 46.7%) with the time to isolation ranging from 16 to 37 days [median 27 days, inter-quartile range (IQR) 22.5-33.5 days]. Patient factors such as days of fever and O. tsutsugamushi IgM antibody titer, t..View full abstract