Journal article

Proteomic analysis of individual human embryos to identify novel biomarkers of development and viability

MG Katz-Jaffe, DK Gardner, WB Schoolcraft

FERTILITY AND STERILITY | ELSEVIER SCIENCE INC | Published : 2006

Abstract

OBJECTIVE: To develop a method to analyze the proteome of individual human blastocysts and identify differentially expressed proteins prior to implantation. DESIGN: Experimental study. SETTING: Research environment. PATIENT(S): Couples undergoing infertility treatment donated with consent cryopreserved human cleavage-stage embryos for research. INTERVENTION(S): Individual embryos were extracted and analyzed by time-of-flight mass spectrometry. MAIN OUTCOME MEASURE(S): The protein expression profiles of individual embryos. RESULT(S): Differential protein expression profiles were observed between early and expanded blastocysts, as well as between developing blastocysts and degenerate embryos. ..

View full abstract

University of Melbourne Researchers