Journal article

Vitrification of mouse pronuclear oocytes with no direct liquid nitrogen contact

MG Larman, CB Sheehan, DK Gardner

REPRODUCTIVE BIOMEDICINE ONLINE | ELSEVIER SCI LTD | Published : 2006

Abstract

The ability to routinely cryopreserve human oocytes and embryos represents a significant advancement in the field of assisted reproductive technology. Although the method of slow freezing is commonly employed, research on the alternative technique of vitrification is promising. Vitrification involves incubation of the cell in a cryoprotectant rich solution, which permits a glass-like state to occur almost instantaneously in liquid nitrogen. A number of different techniques have been invented for holding oocytes and embryos in the cryoprotectant solution during rapid vitrification and subsequent storage. Most of these involve direct contact with liquid nitrogen. Recently, concerns have been r..

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University of Melbourne Researchers