Journal article

Effects of oxygen tension on the establishment and lactate dehydrogenase activity of murine embryonic stem cells

J Gibbons, E Hewitt, DK Gardner

CLONING AND STEM CELLS | MARY ANN LIEBERT, INC | Published : 2006

Abstract

The lack of a standardized culture environment for establishment of embryonic stem cell lines has hindered the orchestrated differentiation of cells and the application of this technology. Oxygen concentration has a profound effect on proliferation and differentiation of many cell types. This study tested the hypothesis that establishment dynamics, lactate dehydrogenase (LDH) isoforms, and mRNA expression patterns would be affected by the oxygen tension in the culture environment. Recovered (day 4) murine blastocysts were cultured in a gas environment of 6% CO(2) and either 20% or 5% O(2) (balance supplemented with N(2)). More (p < 0.05) blastocysts produced outgrowths in the low (79.3 +/- 0..

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University of Melbourne Researchers