Analysis of protein expression (secretome) by human and mouse preimplantation embryos

Abstract

Objective: To analyze the protein production into the surrounding medium (secretome) of both human and mouse embryos and correlate these findings with ongoing blastocyst development. Along with improvements in culture systems, there is renewed focus on the development of noninvasive viability assays in human IVF. Because the majority of biologic functions are carried out by proteins, it is important to study the dynamics of the proteome during embryonic development and its response to both internal and external stimuli. Design: Experimental study. Setting: Research laboratory. Patient(s): Couples undergoing infertility treatment donated with consent spent culture media for research. Interven..