Journal article

A minimal binding footprint on CD1d-glycolipid is a basis for selection of the unique human NKT TCR

Kwok S Wun, Natalie A Borg, Lars Kjer-Nielsen, Travis Beddoe, Ruide Koh, Stewart K Richardson, Meena Thakur, Amy R Howell, James P Scott-Browne, Laurent Gapin, Dale I Godfrey, James McCluskey, Jamie Rossjohn

JOURNAL OF EXPERIMENTAL MEDICINE | ROCKEFELLER UNIV PRESS | Published : 2008

Abstract

Although it has been established how CD1 binds a variety of lipid antigens (Ag), data are only now emerging that show how alphabeta T cell receptors (TCRs) interact with CD1-Ag. Using the structure of the human semiinvariant NKT TCR-CD1d-alpha-galactosylceramide (alpha-GalCer) complex as a guide, we undertook an alanine scanning mutagenesis approach to define the energetic basis of this interaction between the NKT TCR and CD1d. Moreover, we explored how analogues of alpha-GalCer affected this interaction. The data revealed that an identical energetic footprint underpinned the human and mouse NKT TCR-CD1d-alpha-GalCer cross-reactivity. Some, but not all, of the contact residues within the Jal..

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