Journal article

Immunodominance hierarchies and gender bias in direct T-CD8-cell alloreactivity

NA Mifsud, AW Purcell, W Chen, R Holdsworth, BD Tait, J McCluskey

AMERICAN JOURNAL OF TRANSPLANTATION | WILEY | Published : 2008

Abstract

Allogeneic solid organ transplantation often occurs across multiple donor-recipient HLA mismatches with consequent risk of allograft rejection. However, there is growing evidence that not all HLA mismatches are equivalent in their stimulation of allogeneic T cells making it important to determine which of these might be more significant as predictors of allograft rejection. To this end, we used defined antigen-presenting cell (APC) transfectants expressing single MHC-I allotypes as target cells that could discriminate the relative contribution of individual mismatched MHC-I allotypes to direct T-cell alloreactivity. We demonstrate remarkably reproducible patterns of immunodominance in reacti..

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