Journal article

Mixed-linkage (1 -> 3), (1 -> 4)-beta-D-glucan is not unique to the poales and is an abundant component of Equisetum arvense cell walls

Iben Sorensen, Filomena A Pettolino, Sarah M Wilson, Monika S Doblin, Bo Johansen, Antony Bacic, William GT Willats

Plant Journal | WILEY | Published : 2008


Mixed-linkage (1-->3),(1-->4)-beta-D-glucan (MLG) is widely considered to be a defining feature of the cell walls of plants in the Poales order. However, we conducted an extensive survey of cell-wall composition in diverse land plants and discovered that MLG is also abundant in the walls of the horsetail Equisetum arvense. MALDI-TOF MS and monosaccharide linkage analysis revealed that MLG in E. arvense is an unbranched homopolymer that consists of short blocks of contiguous 1,4-beta-linked glucose residues joined by 1,3-beta linkages. However, in contrast to Poaceae species, MLG in E. arvense consists mostly of cellotetraose rather than cellotetriose, and lacks long 1,4-beta-linked glucan bl..

View full abstract