Journal article

Branched and linear lipopeptide vaccines have different effects on primary CD4( ) and CD8( ) T-cell activation but induce similar tumor-protective memory CD8( ) T-cell responses

Adriana Baz, Kathy Buttigieg, Weiguang Zeng, Michael Rizkalla, David C Jackson, Penny Groves, Anne Kelso

VACCINE | ELSEVIER SCI LTD | Published : 2008

Abstract

We compared murine T-cell responses to synthetic lipopeptide vaccines in which the TLR2 ligand Pam(2)Cys was attached to co-linear CD4+ and CD8+ T-cell epitopes of ovalbumin (OVA) in a linear or branched configuration. Mice received OVA-specific transgenic CD8+ and CD4+ T-cells followed by one injection of vaccine. Although the branched lipopeptide was more potent in activating OVA-specific CD4+ and CD8+ T-cells in the primary response, both vaccines induced cytolytic T lymphocytes (CTL) that expressed perforin, granzyme A-C, and IFN-gamma mRNAs and conferred long-term protection of most mice against challenge with OVA-expressing tumor cells. OVA epitope display was reduced in tumors that de..

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