Journal article

Dendritic cell acquisition of epitope cargo mediated by simple cationic peptide structures

Brendon Y Chua, Emily M Eriksson, Daniel P Poole, Weiguang Zeng, David C Jackson

Peptides | ELSEVIER SCIENCE INC | Published : 2008


In this study we evaluate the uptake by murine dendritic cells (DCs) of different synthetic, branched cationic peptide structures with a view to facilitating peptide epitope delivery. The level of cell uptake by fluorescenated peptides was measured by flow cytometry following quenching of extracellular fluorescence with trypan blue. Branched peptides containing either N-terminal arginine or N-terminal lysine residues were able to mediate cell entry but the peptide containing four arginine residues in a branching configuration (R4) was found to be superior not only to other branched peptides in translocating to the cell interior and also to a peptide containing four arginine residues arranged..

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