Local expression of IDO, either alone or in combination with CD40Ig, IL10 or CTLA4Ig, inhibits indirect xenorejection responses
William R Mulley, Yu Din Li, Janet L Wee, Natalie Dodge, Dale Christiansen, Charmaine Simeonovic, Francesco L Ierino, Mauro S Sandrin
XENOTRANSPLANTATION | WILEY | Published : 2008
BACKGROUND: To overcome cell-mediated xenorejection by transgenic expression of immunomodulatory molecules by a graft, it is likely that expression of multiple molecules will be required. Previous studies support the use of the immunomodulatory agents indoleamine 2,3-dioxygenase (IDO), CD40Ig, interleukin 10 (IL10), and CTLA4Ig for suppression of rejection responses. We examined the effects of local expression of these molecules by a porcine cell line (PIEC) on indirect murine xenorejection responses in vitro and in vivo. METHODS: The PIEC stable lines expressing IDO, CD40Ig, and IL10 as single molecules were generated. In addition, PIEC lines expressing IDO with either CD40Ig, IL10 or CTLA4..View full abstract
This work was supported by funds obtained from the National Health and Medical Research Council of Australia.