Journal article

Gene expression analysis in absence epilepsy using a monozygotic twin design

Ingo Helbig, Nicholas A Matigian, Lata Vadlamudi, Kate M Lawrence, Marta A Bayly, Sharon M Bain, Dileepa Diyagama, Ingrid E Scheffer, John C Mulley, Andrew J Holloway, Leanne M Dibbens, Samuel F Berkovic, Nicholas K Hayward

Epilepsia | BLACKWELL PUBLISHING | Published : 2008

Abstract

PURPOSE: To identify genes involved in idiopathic absence epilepsies by analyzing gene expression using a monozygotic (MZ) twin design. METHODS: Genome-wide gene expression in lymphoblastoid cell lines (LCLs) was determined using microarrays derived from five discordant and four concordant MZ twin pairs with idiopathic absence epilepsies and five unaffected MZ twin pairs. Gene expression was analyzed using three strategies: discordant MZ twins were compared as matched pairs, MZ twins concordant for epilepsy were compared to control MZ twins, and a singleton design of affected versus unaffected MZ twin individuals was used irrespective of twin pairing. An overlapping gene list was generated f..

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