Journal article

Transforming growth factor-beta(1) differentially mediates fibronectin and inflammatory cytokine expression in kidney tubular cells

Weier Qi, Xinming Chen, John Holian, Ellein Mreich, Stephen Twigg, Richard E Gilbert, Carol A Pollock

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | AMER PHYSIOLOGICAL SOC | Published : 2006

Abstract

Transforming growth factor-beta(1) (TGF-beta(1)) is not only an important fibrogenic but also immunomodulatory cytokine in the human kidney. We have recently demonstrated that TGF-beta(1) induces interleukin-8 (IL-8), macrophage chemoattractant protein-1 (MCP-1), and fibronectin production in renal proximal tubular (HK-2) cells. However, the unique dependence of IL-8, MCP-1, and fibronectin on TGF-beta(1) expression is unknown. The TGF-beta(1) gene was effectively silenced in HK-2 cells using small-interference (si) RNA. Basal secretion of IL-8 and MCP-1 decreased (both P < 0.05) but, paradoxically, fibronectin increased (P < 0.05) in TGF-beta(1)-silenced cells compared with cells transfecte..

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