Journal article

Terminal deoxynucleotidyltransferase is required for the establishment of private virus-specific CD8( ) TCR repertoires and facilitates optimal CTL responses

Katherine Kedzierska, Paul G Thomas, Vanessa Venturi, Miles P Davenport, Peter C Doherty, Stephen J Turner, Nicole L La Gruta

JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 2008

Abstract

Virus-immune CD8(+) TCR repertoires specific for particular peptide-MHC class I complexes may be substantially shared between (public), or unique to, individuals (private). Because public TCRs can show reduced TdT-mediated N-region additions, we analyzed how TdT shapes the heavily public (to D(b)NP(366)) and essentially private (to D(b)PA(224)) CTL repertoires generated following influenza A virus infection of C57BL/6 (B6, H2(b)) mice. The D(b)NP(366)-specific CTL response was virtually clonal in TdT(-/-) B6 animals, with one of the three public clonotypes prominent in the wild-type (wt) response consistently dominating the TdT(-/-) set. Furthermore, this massive narrowing of TCR selection f..

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