Journal article

Antigen-specific ANCA ELISAs have different sensitivities for active and treated vasculitis and for nonvasculitic disease

Michelle Trevisin, Wendy Pollock, Wayne Dimech, Joy Melny, Bill Paspaliaris, David Gillis, Richard Wong, Judy Savige

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | AMER SOC CLINICAL PATHOLOGY | Published : 2008

Abstract

This study evaluated the performance of 12 assays for antineutrophil cytoplasmic antibodies (ANCA) directed against proteinase 3 (PR3) and myeloperoxidase (MPO) in 55 active and 68 treated cases of vasculitis and in nonvasculitic disease. It examined within- and between-assay precision; binding curves, binding levels, and interassay consistency; and sensitivity, specificity, and receiver operating characteristic analysis. All assays were highly sensitive for active vasculitis (median, 94%; range, 91%-96%), but sensitivities were more varied in treated disease (median, 69%; range, 57%-82%). Binding curves and binding levels were also very variable in PR3-ANCA and MPO-ANCA assays. This has imp..

View full abstract