Delivery of immunotherapy with peptide-pulsed blood in macaques
Robert De Rose, Caroline S Fernandez, Liyen Loh, Viv Peut, Rosemarie D Mason, Sheilajen Alcantara, Jeanette Reece, Stephen J Kent
VIROLOGY | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2008
Simple and effective delivery methods for cellular immunotherapies are needed. We assessed ex vivo pulsing of overlapping SIV Gag 15mer peptides onto either whole blood or PBMC in 15 randomly assigned SIV-infected macaques. Both delivery methods were safe and immunogenic, stimulating high levels of broad and polyfunctional Gag-specific CD4 and CD8 T cells. Delivery of overlapping Gag peptides via either whole blood or PBMC is suitable for clinical evaluation.
This work is supported in part by a grant from the Phillip T. and Susan M. Ragon Foundation.