Quantification of Simian Immunodeficiency Virus Cytotoxic T Lymphocyte Escape Mutant Viruses
Liyen Loh, Stephen J Kent
AIDS RESEARCH AND HUMAN RETROVIRUSES | MARY ANN LIEBERT, INC | Published : 2008
Escape from cytotoxic T-lymphocyte (CTL) pressure is common in HIV-1 infection of humans and simian immunodeficiency virus (SIV) infections of macaques. CTL escape typically incurs a fitness cost as reversion back to wild-type can occur upon transmission. We utilized sequence-specific primers and DNA probes with real-time polymerase chain reaction (PCR) to sensitively and specifically track wild-type and escape mutant viremia at the Mane-A*17-restricted SIV Gag(371379) epitope AF9 in pigtail macaques. The generation of minor escape mutant populations is detected by the real-time PCR 2 weeks earlier than observed using standard sequencing techniques. We passaged the AF9 CTL escape mutant viru..View full abstract
Awarded by Australian NHMRC
We thank Miles Davenport, Janka Petravic, and Jane Batten for helpful advice. Supported by Australian NHMRC grants 299907, 251643, and 454553.