Journal article
Grhl3 and Lmo4 play coordinate roles in epidermal migration
NR Hislop, J Caddy, SB Ting, A Auden, S Vasudevan, SL King, GJ Lindeman, JE Visvader, JM Cunningham, SM Jane
Developmental Biology | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2008
Abstract
In addition to its role in formation of the epidermal barrier, the mammalian transcription factor Grainy head-like 3 (Grhl3) is also essential for neural tube closure and wound repair, processes that are dependent in part on epidermal migration. Here, we demonstrate that the LIM-only domain protein, LMO4 serves as a functional partner of GRHL3 in its established roles, and define a new cooperative role for these factors in another developmental epidermal migration event, eyelid fusion. GRHL3 and LMO4 interact biochemically and genetically, with mutant mice exhibiting fully penetrant exencephaly, thoraco-lumbo-sacral spina bifida, defective skin barrier formation, and a co-incident eyes-open-..
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Funding Acknowledgements
We thank Lan Ta, Erik Dressler and members of the WEHI and BIO21 Animal Set-vices Facilities for assistance with the mouse lilies. We also thank Lin-lin Zhao for excellent technical assistance. S.M.J. is a Principal Research Fellow of the Australian National Health and Medical Research Council (NHMRC). S.B.T. was supported by the Cancer Council of Australia. The work was supported by Project Grants from the Australian NHMRC, and a Grant from the March of Dimes Foundation. Note: After the submission of this manuscript, Yu et al., described a role for Grhl3 alone in formation of the epidermal leading edge during eyelid Closure. Yu, Z., et al., 2008. Grainyhead-like factor Get1/Grhl3 regulates formation of the epidermal leading edge during eyelid closure. Dev Biol., in press.