Journal article

Immunoblotting using multiple antigens is essential to demonstrate the true risk of Helicobacter pylori infection for gastric cancer

H Mitchell, DR English, F Elliott, M Gengos, JH Barrett, GG Giles, D Forman



BACKGROUND: Enzyme-linked immunosorbent assays (ELISAs) for detection of Helicobacter pylori infection, using IgG antibodies, may significantly underestimate the association with gastric cancer. AIM: To compare associations between H. pylori and cardia (CGC) and noncardia gastric cancer (NCGC) using ELISA and immunoblotting and determine the effect of atrophic gastritis on detection. METHODS: Nested case-control study within the Melbourne Collaborative Cohort Study. Helicobacter pylori antibodies were detected in subjects with CGC (n = 18), NCGC (n = 34) and controls (n = 69 and 134 respectively) using ELISA (pylori DTect) and immunoblot (Helicoblot 2.1). Pepsinogen I levels were measured us..

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Awarded by Medical Research Council

Funding Acknowledgements

The MCCS is made possible by the contribution of many people including the original investigators and the diligent team which recruited the participants and continue working on follow-up. The authors express their gratitude to the many thousands of Melbourne residents who continue to participate in the study. Declaration of personal interests: Cancer Research UK supports the work of FE and JHB. Declaration of funding interests: Funded by grants from the Australian National Health and Medical Research Council grants (209057, 251533). Cohort recruitment was funded by The Cancer Council Victoria and VicHealth.