Journal article

High-resolution X-ray imaging of Plasmodium falciparum-infected red blood cells

Garth J Williams, Eric Hanssen, Andrew G Peele, Mark A Pfeifer, Jesse Clark, Brian Abbey, Guido Cadenazzi, Martin D de Jonge, Stefan Vogt, Leann Tilley, Keith A Nugent

CYTOMETRY PART A | WILEY-LISS | Published : 2008

Abstract

Methods for imaging cellular architecture and ultimately macromolecular complexes and individual proteins, within a cellular environment, are an important goal for cell and molecular biology. Coherent diffractive imaging (CDI) is a method of lensless imaging that can be applied to any individual finite object. A diffraction pattern from a single biological structure is recorded and an iterative Fourier transform between real space and reciprocal space is used to reconstruct information about the architecture of the sample to high resolution. As a test system for cellular imaging, we have applied CDI to an important human pathogen, the malaria parasite, Plasmodium falciparum. We have employed..

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Grants

Awarded by U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences


Funding Acknowledgements

Grant sponsors: Australian Research Council, Australian National Health and Medical Research Council, and the Australian Synchrotron Research Program.Use of the Advanced Photon Source was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, under Contract No. DE-AC0206CH11357. We thank Alan Cowman, Walter and Eliza Hall Institute for supplying transfected parasites.