Granzyme K Expressing Cytotoxic T Lymphocytes Protects Against Influenza Virus in Granzyme AB(-/-) Mice
Misty R Jenkins, Joseph A Trapani, Peter C Doherty, Stephen J Turner
VIRAL IMMUNOLOGY | MARY ANN LIEBERT, INC | Published : 2008
Granzyme (grz) AB(-/-) H2(b) mice generate numerically normal cytotoxic T lymphocyte (CTL) responses to the prominent influenza A virus D(b) NP(366) and D(b) PA(224) epitopes and terminate the infectious process in the pneumonic lung with the same kinetics as the WT controls. Though grz B protein expression is fully compromised, there is only a partial effect on the level of CTL activity measured in a classical, short-term (51)Cr release assay. Single-cell polymerase chain reaction (PCR) analysis of both highly activated effector and "resting" memory CD8(+) T cells from influenza A virus-infected grzAB(-/-) mice showed a high prevalence of grzK mRNA(+) expression in tetramer (tet)(+) CTLs as..View full abstract
We thank Dr. Nigel Waterhouse for critical review and discussion and Ken Field for cell sorting. This work was supported by an Australian Postgraduate Scholarship (M. R. J.), a National Health and Medical Research Council Burnet Award (P. C. D), a Victorian Government Science, Technology and Innovation grant (P. C. D), NHMRC program grant and fellowship (J. A. T.), a Pfizer Senior Research Fellowship (S. J. T.), and a Melbourne University Early Career Researcher grant (S. J. T).