Journal article
Granzyme K expressing cytotoxic T lymphocytes protects against influenza virus in granzyme AB-/- mice
MR Jenkins, JA Trapani, PC Doherty, SJ Turner
Viral Immunology | Published : 2008
Abstract
Granzyme (grz) AB-/- H2b mice generate numerically normal cytotoxic T lymphocyte (CTL) responses to the prominent influenza A virus Db NP366 and Db PA224 epitopes and terminate the infectious process in the pneumonic lung with the same kinetics as the WT controls. Though grz B protein expression is fully compromised, there is only a partial effect on the level of CTL activity measured in a classical, short-term 51Cr release assay. Single-cell polymerase chain reaction (PCR) analysis of both highly activated effector and "resting" memory CD8+ T cells from influenza A virus-infected grzAB-/- mice showed a high prevalence of grzK mRNA+ expression in tetramer (tet)+ CTLs as was found in WT mice...
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Funding Acknowledgements
We thank Dr. Nigel Waterhouse for critical review and discussion and Ken Field for cell sorting. This work was supported by an Australian Postgraduate Scholarship (M. R. J.), a National Health and Medical Research Council Burnet Award (P. C. D), a Victorian Government Science, Technology and Innovation grant (P. C. D), NHMRC program grant and fellowship (J. A. T.), a Pfizer Senior Research Fellowship (S. J. T.), and a Melbourne University Early Career Researcher grant (S. J. T).