Journal article

Blood-stage Plasmodium infection induces CD8( ) T lymphocytes to parasite-expressed antigens, largely regulated by CD8 alpha( ) dendritic cells

Rachel J Lundie, Tania F de Koning-Ward, Gayle M Davey, Catherine Q Nie, Diana S Hansen, Lei Shong Lau, Justine D Mintern, Gabrielle T Belz, Louis Schofield, Francis R Carbone, Jose A Villadangos, Brendan S Crabb, William R Heath

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | NATL ACAD SCIENCES | Published : 2008

Abstract

Although CD8(+) T cells do not contribute to protection against the blood stage of Plasmodium infection, there is mounting evidence that they are principal mediators of murine experimental cerebral malaria (ECM). At present, there is no direct evidence that the CD8(+) T cells mediating ECM are parasite-specific or, for that matter, whether parasite-specific CD8(+) T cells are generated in response to blood-stage infection. To resolve this and to define the cellular requirements for such priming, we generated transgenic P. berghei parasites expressing model T cell epitopes. This approach was necessary as MHC class I-restricted antigens to blood-stage infection have not been defined. Here, we ..

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Grants

Funding Acknowledgements

We thank Lynn Buckingham, Chrystal Smith, Mary Camilleri, Fiona Kupresanin, Jiang-Li Tan, and Jane Langley for assistance, and Drs. Chris Janse and Andy Waters (Leiden University Medical Centre, The Netherlands) for the P. berghei plasmid pGFi The National Health and Medical Research Council of Australia supported this work. R.J.L. was supported by an Australian Postgraduate Award and the Cooperative Research Centre for Vaccine Technology. L.S., G.T.B., B.S.C., and W.R.H. are International Research Scholars of the Howard Hughes Medical Institute (Chevy Chase, MD)