Journal article

Exploring 'best practice' for nucleic acid detection of Neisseria gonorrhoeae

David M Whiley, Suzanne M Garland, Geoffrey Harnett, Gary Lum, David W Smith, Sepehr N Tabrizi, Theo P Sloots, John W Tapsall



Nucleic acid detection tests (NADT) have considerable benefits for the detection of Neisseria gonorrhoeae (GC), including high sensitivity across a range of specimen types and use under widely differing settings and conditions. However, sexual health practitioners and others who use data generated by NADT for GC should be aware of some important limitations of these tests. False-positive results caused by cross reaction with commensal Neisseria species have been observed in many assays, and have lead to unacceptably low positive-predictive values in some patient populations. Further, false-negative results can be caused by GC sequence variation, with some gonococci lacking certain NADT targe..

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