Decreased IL-10 and IL-13 production and increased CD44(hi) T cell recruitment contribute to Leishmania major immunity induced by non-persistent parasites
Lukasz Kedzierski, Joan M Curtis, Peter C Doherty, Emanuela Handman, Katherine Kedzierska
EUROPEAN JOURNAL OF IMMUNOLOGY | WILEY-BLACKWELL | Published : 2008
Leishmaniasis is currently classified as category 1 disease, i.e. emerging and uncontrolled. Since the importance of persistent infection for maintaining an effective long-lasting protective response is controversial, the present study asks whether immunisation with non-persistent parasites leads to protection against Leishmania infection and to the recruitment of T cells of a specific phenotype. Our study shows that vaccination of susceptible BALB/c mice with live Leishmania major phosphomannomutase-deficient parasites, which are avirulent and non-persistent in vivo, leads to protection against infection. Immunisation with phosphomannomutase-deficient parasites neither leads to differences ..View full abstract
We sincerely thank Dr Diana Hansen and Ms Catherine Nie for helpful suggestions and immunological reagents, Dr Axel Kallies and Mr Paul Whitney for their expertise with ICS. This work was funded by the National Health and Medical Research Council and the NHMRC/ARC Network for Parasitology (L. K. and E. H.). K K. is an NHMRC RD Wright Research Fellow. P.C.D. is an NHMRC Burnet Fellow.